We used genetic crosses with the mixed brown stock to create ST and SR flies that were genetically similar except for the X-chromosome (electronic supplementary material, figure S2). Me and SRNy) and four wild-type stocks (MI, New york1, Nyc2, PEI). Males used in the experiment carry an ST or SR X-chromosome from the tester stock, and females https://datingranking.net/nl/alua-overzicht/ are heterozygous at the X-chromosome, with one b X-chromosome and the other from the tester ST or SR stock (electronic supplementary material, figure S2). Flies were collected as virgins and stored 10 flies vial ?1 on regular culture food. At one week of age, flies were transferred to vials containing a blended mushroom-agar food. Survival was assayed every 1–2 days, and the flies were transferred to fresh food every 4 days. Survival analyses used a Cox proportional hazard model with P roc P hreg in SAS v. 9.3 (SAS Institutes), with SR carrier status and line nested within SR carrier status as fixed effect variables, and the vial as a random effect variable. Analyses were completed separately for females and males.
(d) Lady lover discrimination
We tested for virgin female discrimination against mating with SR males using both no-choice and choice assays. First, we conducted no-choice trials with SRMe and ST males. Using a series of genetic crosses, we created morphologically indistinguishable males that were either SRMe personally or ST with an otherwise similar genetic background (electronic supplementary material, figure S3). „These crosses was indeed completed by themselves towards several SR stocks (SR“ weiterlesen